(N/A) The biochemical events in the root nodule of a pulse plant involve biological nitrogen fixation,primarily catalyzed by the enzyme $Nitrogenase$.
$1$. The enzyme $Nitrogenase$ is a $Mo-Fe$ protein that catalyzes the conversion of atmospheric nitrogen $(N_2)$ into ammonia $(NH_3)$.
$2$. The reaction is: $N_2 + 8e^- + 8H^+ + 16ATP \rightarrow 2NH_3 + H_2 + 16ADP + 16Pi$.
$3$. The end product of this reaction is ammonia $(NH_3)$.
$4$. The fate of ammonia: At physiological $pH$,ammonia is protonated to form ammonium ions $(NH_4^+)$. While $NH_4^+$ is toxic to plants in high concentrations,it is rapidly incorporated into amino acids through two main pathways:
$(i)$ Reductive amination: $NH_4^+$ reacts with $\alpha$-ketoglutaric acid to form glutamic acid.
(ii) Transamination: The transfer of an amino group from one amino acid to the keto group of a keto acid,catalyzed by transaminases.